Preservation of ATP in Hypersaline Environments

Preservation of ATP in hypersaline environments.

High concentrations of particulate ATP were found in the anoxic brines of the Orca Basin and East Flower Garden, Gulf of Mexico. Other measurements indicative of growth and respiration suggested that the microbial community in the brines was inactive, but somehow the ATP associated with the cells persisted. Conceivably, when cells growing just above the interface sank into the brine, the increa...

Biodegradation of petroleum hydrocarbons in hypersaline environments

Literature on hydrocarbon degradation in extreme hypersaline media presents studies that point to a negative effect of salinity increase on hydrocarbonoclastic activity, while several others report an opposite tendency. Based on information available in the literature, we present a discussion on the reasons that justify these contrary results. Despite the fact that microbial ability to metaboli...

Pyruvate: A key Nutrient in Hypersaline Environments?

Some of the most commonly occurring but difficult to isolate halophilic prokaryotes, Archaea as well as Bacteria, require or prefer pyruvate as carbon and energy source. The most efficient media for the enumeration and isolation of heterotrophic prokaryotes from natural environments, from freshwater to hypersaline, including the widely used R2A agar medium, contain pyruvate as a key ingredient....

Characterization of New Viruses from Hypersaline Environments

Preservation Environments

The long-term preservation of digital entities requires mechanisms to manage the authenticity of massive data collections that are written to archival storage systems. Preservation environments impose authenticity constraints and manage the evolution of the storage system technology by building infrastructure independent solutions. This seeming paradox, the need for large archives, while avoidi...